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lightcycler probe design software 2 0  (Roche)


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    Structured Review

    Roche lightcycler probe design software 2 0
    Lightcycler Probe Design Software 2 0, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lightcycler+probe+design+software+2+0/LightCycler+480+System/bio_rxiv__2025__09__28__679053-278-9-14
    Average 99 stars, based on 6 article reviews
    lightcycler probe design software 2 0 - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: A Poly-Glutamine Region in the Drosophila VAChT Dictates Fill-Level of Cholinergic Synaptic Vesicles
    Article Snippet: .. PCR primers were designed with the aid of LightCycler Probe Design Software 2.0 (v1.0; Roche). ..

    Software:

    Article Title: A Poly-Glutamine Region in the Drosophila VAChT Dictates Fill-Level of Cholinergic Synaptic Vesicles
    Article Snippet: .. PCR primers were designed with the aid of LightCycler Probe Design Software 2.0 (v1.0; Roche). ..

    Article Title: Isolation and characterization of a human cementocyte-like cell line, HCY-23.
    Article Snippet: Total RNA was obtained using the RNeasy system (Qiagen, Valencia, USA) following the manufacturer’s recommendations, and complimentary DNA (cDNA) synthesized from 1 μg of total RNA (Transcriptor First Strand cDNA Synthesis Kit, Roche Diagnostic Co., Mannheim, Germany), following the recommended protocol. .. Primer sequences were obtained with the LightCycler® Probe Design Software 2.0 (Roche Diagnostics GmbH, Germany) and are listed in Table 2. .. Quantitative real-time PCR (qPCR) reactions were optimized for each primer and water was used as a negative control for the reactions. qPCR reactions were performed using the SYBR Green System (FastStart Essential DNA Green Master; Roche Diagnostic Co., Indianapolis, USA) on the LightCycler 480 instrument (Roche Diagnostics GmbH, Netherland).

    Article Title: Combining a Mutant Allele of FAD2-1A with HD Improves the ω-6/ω-3 Ratio in Soybeans
    Article Snippet: .. The SimpleProbe was designed using the LightCycler Probe Design Software 2.0 (version 1, Roche Applied Sciences, Penzberg, Germany) based on the position of mutation. ..

    Article Title: Gut alterations in a chronic kidney disease rat model with diet-induced vascular calcification.
    Article Snippet: First strand cDNA was synthesized, from 1 mg RNA, at 37 °C for 60 min with Omniscript Reverse Transcription kit (205111; Qiagen). .. Specific primers listed in Fig. S1 were designed with LIGHTCYCLER Probe Design Software 2.0 (version 1.0.R.36; Roche) from mRNA sequences found in the NCBI nucleotide database and validated experimentally. .. Primers were synthesized by Integrated DNA Technologies (Leuven, Belgium).

    Article Title: miR155 triplicated in Down syndrome regulates hippocampal GABAergic neurogenesis in Alzheimer’s disease
    Article Snippet: After washing 3 times with TBST for 10 min each, membranes were developed with ECL Western blotting substrate (Pierce, Rockford, IL, USA) and were imaged on ChemiDoc MP imaging system (Bio-Rad). .. To reduce non-specific signals, primer sequences were designed using LightCycler Probe Design Software 2.0 (Roche, Germany) such that they spanned splice junctions, and they are listed in Supplemental table S2 and validated in pga.mgh.harvard.edu/primerbank/. .. The integrated density of immunoreactive bands was measured using Fiji software (ImageJ).

    Article Title: Development of a rapid detection system for fluoroquinolone-resistant Corynebacterium macginleyi.
    Article Snippet: We previously reported an increase in high-level fluoroquinolone-resistant Corynebacterium macginleyi isolates from conjunctival swab specimens and investigated the relationship between fluoroquinolone resistance and mutations in the quinolone resistance-determining region (QRDR) of the gene encoding DNA gyrase subunit A (gyrA).. The purpose of this study was to develop a rapid system for detecting fluoroquinolone-resistant C. macginleyi using TaqMan single nucleotide polymorphism (SNP) genotyping assays.. The study included 49 strains of C. macginleyi, of which 45 strains were isolated from patients awaiting ophthalmic surgery and 4 strains were from patients with presumed keratoconjunctivitis.

    Article Title: A wound inducible chalcone synthase gene from Dysoxylum gotadhora ( DbCHS ) regulates flavonoid biosynthesis.
    Article Snippet: Chalcone synthase (CHS) is a type III polyketide synthase and a key enzyme of the phenylpropanoid pathway that generates precursors for flavonoid biosynthesis.. The tree species D. gotadhora is known for having an abundance of rohitukine, which has anti-inflammatory and immune-modulating effects.. In this study, we used the leaves of D. gotadhora to clone CHS gene (DbCHS).

    Mutagenesis:

    Article Title: Combining a Mutant Allele of FAD2-1A with HD Improves the ω-6/ω-3 Ratio in Soybeans
    Article Snippet: .. The SimpleProbe was designed using the LightCycler Probe Design Software 2.0 (version 1, Roche Applied Sciences, Penzberg, Germany) based on the position of mutation. ..

    Article Title: Novel Allele of FAD2-1A from an EMS-Induced Mutant Soybean Line (PE529) Produces Elevated Levels of Oleic Acid in Soybean Oil
    Article Snippet: .. Simpleprobe, obtained from Fluoresentric, Inc. (Park City, UT, USA), was designed using the Lightcycler Probe Design Software 2.0 (Version 1, Roche Applied Sciences, Penzberg, Germany) by inputting the target region of the sequence with a single highlighted mutant position. ..

    Sequencing:

    Article Title: Novel Allele of FAD2-1A from an EMS-Induced Mutant Soybean Line (PE529) Produces Elevated Levels of Oleic Acid in Soybean Oil
    Article Snippet: .. Simpleprobe, obtained from Fluoresentric, Inc. (Park City, UT, USA), was designed using the Lightcycler Probe Design Software 2.0 (Version 1, Roche Applied Sciences, Penzberg, Germany) by inputting the target region of the sequence with a single highlighted mutant position. ..

    Gene Expression:

    Article Title: A wound inducible chalcone synthase gene from Dysoxylum gotadhora ( DbCHS ) regulates flavonoid biosynthesis.
    Article Snippet: Chalcone synthase (CHS) is a type III polyketide synthase and a key enzyme of the phenylpropanoid pathway that generates precursors for flavonoid biosynthesis.. The tree species D. gotadhora is known for having an abundance of rohitukine, which has anti-inflammatory and immune-modulating effects.. In this study, we used the leaves of D. gotadhora to clone CHS gene (DbCHS).



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